@article{JTD1018,
author = {Haiyan Liu and Hai Wang and Mao Huang and Yaning Mei and Bing Gu and Rui Wu and Yiling Huang and Yi Chen and Yanling Xu and Tong Wang},
title = {Analysis of antimicrobial resistance and class 1 integrons among strains from upper respiratory tract of healthy adults},
journal = {Journal of Thoracic Disease},
volume = {5},
number = {2},
year = {2013},
keywords = {},
abstract = {Objective: The distribution and characterization of integrons among opportunistic pathogens from nasopharynx of healthy adults.
Methods: A total of 1,019 nasopharyngeal samples from healthy adults were collected; bacteria were identified by API system; antibiotic susceptibility were tested by K-B method; class 1, 2 and 3 integrons were examined by degenerate primers of the genetic content of integrons were analyzed by PCR and DNA sequencing.
Results: Out of the 1,019 cases, 743 (72.9%) opportunistic pathogens were isolated. The top five commons organism identified were Coagulase-negative staphylococcus (n=404), Staphylococcus aureus (n=109), Haemophilus influenzae (n=74), Streptococcus pneumoniae (n=49) and Klebsiella pneumoniae (n=32). Eight (25.0%) isolates of K. pneumoniae produced the ESBLs. The isolated rates of S. aureus and H. influenzae were decreased with aging. 6.3% (2/32) K. pneumoniae isolates and 16.7% (1/8) Proteus isolates carried class 1 integrons. Among intI1-positive strains, sequencing analysis revealed that one of the integron positive K. pneumoniae isolates harbored gene cassette aadA5-dfrA17. Proteus isolates harbored gene cassette aadA2-dhfrXII.
Conclusions: The results stressed the need for continued surveillance of bacteria from the asymptomatic carriers.},
issn = {2077-6624}, url = {https://jtd.amegroups.org/article/view/1018}
}