@article{JTD125658,
author = {Yikun Chen and Yucheng Liu and Jiayi Shen and Kerong Wang and Jun Jiang and Huazhong Cai and Jiangning Yin},
title = {Glycyrrhizic acid attenuates sepsis-induced acute lung injury by regulating macrophage M2 polarization via PRKCD},
journal = {Journal of Thoracic Disease},
volume = {0},
number = {0},
year = {2026},
keywords = {},
abstract = {Background: This study aimed to investigate the protective effects of glycyrrhizic acid (GA) in sepsis-induced acute lung injury (SI-ALI) and explore the underlying molecular mechanisms, with a focus on macrophage polarization mediated by protein kinase C delta (PRKCD).Methods: A cecal ligation and puncture (CLP) model was used to induce sepsis in mice. GA was administered intraperitoneally at different doses for in vivo intervention, and PRKCD overexpression was employed to validate the mechanism. In vitro, an inflammatory model was established by lipopolysaccharide (LPS) stimulation of RAW 264.7 macrophage cells, followed by GA treatment and PRKCD silencing or overexpression. Hematoxylin and eosin (HE) staining, lung wet-to-dry weight (W/D) ratio measurement, immunohistochemistry, flow cytometry, immunofluorescence, Cell Counting Kit-8 (CCK-8) assay, terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL), Western blot, and enzyme-linked immunosorbent assay (ELISA) were used to assess inflammation, PRKCD expression, and macrophage polarization in lung tissues and cells.Results: GA significantly alleviated CLP-induced lung tissue injury and pulmonary edema (W/D ratio: 4.5±0.8 vs. 6.7±0.8 in the Model group, P},
issn = {2077-6624}, url = {https://jtd.amegroups.org/article/view/125658}
}