How to cite item

Is tail vein injection a relevant breast cancer lung metastasis model?

   
@article{JTD1276,
	author = {Omar M. Rashid and Masayuki Nagahashi and Suburamaniam Ramachandran and Catherine I. Dumur and Julia C. Schaum and Akimitsu Yamada and Tomoyoshi Aoyagi and Sheldon Milstien and Sarah Spiegel and Kazuaki Takabe},
	title = {Is tail vein injection a relevant breast cancer lung metastasis model?},
	journal = {Journal of Thoracic Disease},
	volume = {5},
	number = {4},
	year = {2013},
	keywords = {},
	abstract = {Background: Two most commonly used animal models for studying breast cancer lung metastasis are: lung metastasis after orthotopic implantation of cells into the mammary gland, and lung implantations produced after tail vein (TV) injection of cells. Tail vein injection can produce lung lesions faster, but little has been studied regarding the differences between these tumors, thus, we examined their morphology and gene expression profiles. 
Methods: Syngeneic murine mammary adenocarcinoma, 4T1-luc2 cells, were implanted either subcutaneously (Sq), orthotopically (OS), or injected via TV in Balb/c mice. Genome-wide microarray analyses of cultured 4T1 cells, Sq tumor, OS tumor, lung metastases after OS (LMet), and lung tumors after TV (TVt) were performed 10 days after implantation. 
Results: Bioluminescence analysis demonstrated different morphology of metastases between LMet and TVt, confirmed by histology. Gene expression profile of cells were significantly different from tumors, OS, Sq, TVt or LMet (10,350 probe sets; FDR≤1%; P},
	issn = {2077-6624},	url = {https://jtd.amegroups.org/article/view/1276}
}