@article{JTD15004,
author = {Ge Gong and Xin-Xing Yang and Yanyan Li and Hong-Yu Geng and Zhi-Jian Yang and Lian-Sheng Wang and Hyun Jun Kim and Xin-Zheng Lu},
title = {LncRNA260-specific siRNA targeting IL28RA gene inhibit cardiomyocytes hypoxic/reoxygenation injury},
journal = {Journal of Thoracic Disease},
volume = {9},
number = {8},
year = {2017},
keywords = {},
abstract = { Background: The interleukin 28 receptor alpha (IL28RA) gene was indicated to be associated with apoptosis. However, it was not clear whether long non-coding RNA 260 (lncRNA 260)-specific siRNA targeting IL28RA gene could inhibit hypoxic reoxygenation (H/R) cardiomyocytes injury or not. To explore the mechanisms underlying the protective effects of lncRNA260-specific siRNA-mediated inhibition of IL28RA from H/R injury in car-diomyocytes, the current research was performed.
Methods: The primary neonatal rat cardiomyocytes were transfected with three different pairs of siRNA specific to lncRNA260 targeting IL28RA gene and then were undergone with the conditions simulating H/R injury.
Results: All three groups of cardiomyocytes treated with lncRNA260-specific siRNA experienced significantly decreased levels of lactate dehy-drogenase activity and apoptosis rate relative to the non-treatment and negative control groups (P},
issn = {2077-6624}, url = {https://jtd.amegroups.org/article/view/15004}
}