TY  - JOUR
AU  - Legras, Antoine
AU  - Tallet, Anne
AU  - Didelot, Audrey
AU  - Cazes, Aurélie
AU  - Danel, Claire
AU  - Hin, Angela
AU  - Borie, Raphaël
AU  - Crestani, Bruno
AU  - Castier, Yves
AU  - Bagan, Patrick
AU  - Pimpec-Barthes, Françoise Le
AU  - Riquet, Marc
AU  - Blons, Hélène
AU  - Mordant, Pierre
PY  - 2018
TI  - Clinical and molecular characteristics of unicentric mediastinal Castleman disease
JF  - Journal of Thoracic Disease; Vol 10, No 4 (April 30, 2018): Journal of Thoracic Disease
Y2  - 2018
KW  - 
N2  - Background: Unicentric mediastinal Castleman disease (CD) is a rare condition, poorly characterized due to the small number of cases and the absence of genomic study. We analyzed clinical, radiological, histological and genomic patterns associated with mediastinal CD in a substantial case series.  Methods: We retrospectively reviewed cases of unicentric mediastinal CD managed in 2 French thoracic surgery departments between 1988 and 2012. Clinical, radiological, surgical and pathological data were recorded. On available FFPE blocks we performed mutation screening by next-generation-sequencing, using AmpliSeq™ Cancer Hotspot v2 (Life Technologies) and immunohistochemistry (IHC) (AKT-mTOR pathway).  Results: Eleven patients were identified (mean age 41±15 years, sex-ratio 0.8, median follow-up 78 months). Surgical approach was thoracotomy (n=6), sternotomy (n=4), and VATS (n=1). Additional procedures included thymectomy in three cases, mediastinal lymphadenectomy in two cases, and bilobectomy in one case. One patient presented local relapse as a follicular dendritic cell sarcoma, leading to death 48 months after the first resection. Within 9 patients whose FFPE blocks were available, 2 mutations were found: VHL (p.F119L, 35%, n=1) and JAK3 (p.V718L, 53%, n=1). Phospho-AKT and phospho-mTOR stainings were negative in all cases, whereas phospho-S6RP staining was positive in eight cases, mainly in interfollicular cell cytoplasm.  Conclusions: From this series of patients with unicentric mediastinal CD, we observed 2 cases of potential driver mutations and 8 cases of phospho-S6RP activation not related to AKT-mTOR. Larger studies are required to decipher more precisely the molecular abnormalities and potential therapeutic targets underlying this uncommon condition.
UR  - https://jtd.amegroups.org/article/view/20692