@article{JTD230,
author = {Ismail Cinel and Mustafa Ark and Phillip Dellinger and Tuba Karabacak and Lulufer Tamer and Leyla Cinel and Paul Michael and Shaimaa Hussein and Joseph E. Parrillo and Anand Kumar and Aseem Kumar},
title = {Involvement of Rho kinase (ROCK) in sepsis-induced acute lung injury},
journal = {Journal of Thoracic Disease},
volume = {4},
number = {1},
year = {2011},
keywords = {},
abstract = {Indirect acute lung injury is associated with high morbidity and mortality. We investigated the link between Rho kinase (ROCK) activation and apoptotic cell death in sepsis induced acute lung injury. This hypothesis was tested by administering a specific, selective inhibitor of ROCK (Y-27632) to rats subjected to cecal ligation and puncture (CLP). Rats were randomly divided into 4 groups as; sham-operated, sham + Y-27632, CLP and CLP + Y-27632. Twenty-four hours later, each experiment was terminated and lungs analyzed. Histopathology was assessed by hematoxylin-eosin staining and the presence of apoptosis was evaluated through the TUNEL assay. Pulmonary activity of caspase 3 and ROCK 1 & 2 were measured by western blot. Interstitial edema, severely damaged pulmonary architecture with massive infiltration of the inflammatory cells and an increase in lung tissue TBARS levels as well as 3-NT to total tyrosine ratios were observed in untreated CLP animals. Pretreatment of animals with Y-27632, reduced lung injury in the CLP induced septic rats in each of these parameters of lung injury (p},
issn = {2077-6624}, url = {https://jtd.amegroups.org/article/view/230}
}