@article{JTD34046,
author = {Masahiro Yanagiya and Kentaro Kitano and Takuma Yotsumoto and Daiya Takai and Hiromichi Asahina and Kazuhiro Nagayama and Jun Nakajima},
title = {Effect of normal saline flush injection into a bronchus on lung decellularization},
journal = {Journal of Thoracic Disease},
volume = {11},
number = {12},
year = {2019},
keywords = {},
abstract = {Background: The aim of this study was to evaluate effect of normal saline flush injection into bronchus on creation of decellularized lung scaffolds.
Methods: Pigs were used: 3 lung grafts for decellularization with pre-treatment of normal saline injection into a bronchus, 3 for decellularization without pre-treatment and 3 treated as normal controls. We compared the characteristics of lung scaffolds created by each method.
Results: The pretreatment procedure significantly reduced the DNA content of lung grafts, suggesting effective removal of cellular components. However, this pretreatment also reduced the elastin contents of lung grafts.
Conclusions: Considering this characteristic of saline pretreatment, we must continue to look for better methods to produce ideal decellularized lung grafts.},
issn = {2077-6624}, url = {https://jtd.amegroups.org/article/view/34046}
}