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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of beta-hemolytic streptococci

  
@article{JTD4301,
	author = {Chunmei Zhou and Lili Tao and Bijie Hu and Jian Ma and Xiangru Ye and Shenglei Huang and Yan Ma and Yuzhang Shan},
	title = {Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of beta-hemolytic streptococci},
	journal = {Journal of Thoracic Disease},
	volume = {7},
	number = {4},
	year = {2015},
	keywords = {},
	abstract = {Objective: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as promising technology for species identification. The purpose of this investigation was to compare the performance of MS and the traditional method for identification of beta-hemolytic streptococci (BHS). 
Methods: Clinical BHS isolates were identified by the BD Phoenix SMIC/ID Streptococcal panels, and two MALDI-TOF MS platforms: the VITEK MS and the Bruker MALDI Biotyper systems respectively. In case of discordant results, 16sRNA sequencing was performed to provide the reference ID. 
Results: A total of 96 isolates of BHS were analyzed. Thirty-six isolates (20.8%) were re-tested by BD Phoenix for identification failure; and four isolates (4.2%) were rerun on the Bruker system for low identification score. No isolate need a second run for identification by Vitek MS system. Overall, BD Phoenix, BioTyper and Vitek MS automated system accurately identified 76 strains (79.2%), 91 (94.7%) strains and 92 (95.8%) strains, respectively. 
Conclusions: Our study suggests that MALDI-TOF MS is a superior method to conventional phenotypic methods for BHS identification.},
	issn = {2077-6624},	url = {https://jtd.amegroups.org/article/view/4301}
}