@article{JTD5486,
author = {Xiao-Meng He and Ying Zhou and Ming-Zhen Xu and Yang Li and Hu-Qun Li and Wei-Yong Li},
title = {Effects of long-term smoking on the activity and mRNA expression of CYP isozymes in rats},
journal = {Journal of Thoracic Disease},
volume = {7},
number = {10},
year = {2015},
keywords = {},
abstract = {Background: To investigate the effect of long-term smoking on the activity and mRNA expression of cytochrome P450 (CYP) enzymes.
Methods: Sprague-Dawley rats were exposed to passive smoking 6 cigarettes per day for 180 days. A cocktail solution which contained phenacetin (20 mg/kg), tolbutamide (5 mg/kg), chlorzoxazone (20 mg/kg) and midazolam (10 mg/kg) was given orally to rats. Blood samples were collected at pre-specified time points and the concentrations of probe drugs in plasma were determined by HPLC-MS/MS. The corresponding pharmacokinetic parameters were calculated by DAS 3.0. In addition, real-time RT-PCR was used to analyze the mRNA expression of CYP1A2, CYP2C11, CYP2E1 and CYP3A1 in rat liver.
Results: There were no significant influences of pharmacokinetic profiles of chlorzoxazone in long-term smoking pretreated rats. But many pharmacokinetic profiles of phenacetin, tolbutamide, and midazolam in long-term smoking pretreated rats were affected significantly (P},
issn = {2077-6624}, url = {https://jtd.amegroups.org/article/view/5486}
}