@article{JTD7244,
author = {Likun Hou and Shengxiang Ren and Bo Su and Liping Zhang and Wei Wu and Wei Zhang and Zhengwei Dong and Yan Huang and Chunyan Wu and Gang Chen},
title = {High concordance of ALK rearrangement between primary tumor and paired metastatic lymph node in patients with lung adenocarcinoma},
journal = {Journal of Thoracic Disease},
volume = {8},
number = {6},
year = {2016},
keywords = {},
abstract = {Background: Lung cancer has heterogeneous features. It remains unclear whether ALK rearrangement was distributed heterogeneously in tumor from different anatomic sites. To address this issue, we investigate the concordance of ALK rearrangement between primary tumors and paired metastatic lymph nodes in pulmonary adenocarcinoma patients.
Methods: From Sep 2013 to May 2014, resectable lung adenocarcinoma patients with EGFR wildtype and paired metastatic lymph nodes from Tongji University affiliated Shanghai pulmonary hospital were selected into this study. An auto-mated Ventana ALK with clone D5F3 antibody immunohistochemistry (IHC) and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to detected ALK rearrangement. Discordant cases between IHC and RT-PCR were further validated by fluorescence in situ hybridization (FISH).
Results: A total of 101 patients were enrolled into this study with a median age of 60 years old (range,
35–78 years). ALK rearrangement was found in 20 primary lesions, while in 18 paired metastatic lymph nodes. ALK rearrangement was more frequently happened in younger (P},
issn = {2077-6624}, url = {https://jtd.amegroups.org/article/view/7244}
}