Erratum: Club cell secretory protein 16 promotes cell proliferation and inhibits inflammation and pyroptosis in response to particulate matter 2.5-induced epithelial damage in asthmatic mice

Erratum to: J Thorac Dis 2025;17:753-73.

This article (1) entitled “Club cell secretory protein 16 promotes cell proliferation and inhibits inflammation and pyroptosis in response to particulate matter 2.5-induced epithelial damage in asthmatic mice” (J Thorac Dis 2025;17:753-73, doi: 10.21037/jtd-24-1371) includes some errors in Figures 3,5,7,8 and Figure S2.

In Figure 3, several errors were identified: the TLR4 immunohistochemical staining of control group was unintentionally duplicated in Figure 3A; the β-actin and Cleaved Caspase-1 western blots were incorrectly assembled in Figure 3B.

The original Figure 3 is presented below:

The correct version of Figure 3, containing the correct data for the TLR4 immunohistochemical staining of the control group in Figure 3A, the β-actin and the Cleaved Caspase-1 western blots in Figure 3B, is shown below.

Figure 3 Lung injury induced by OVA and exposure to PM_2.5. (A) IHC staining (n=10 in each group). (B) Western blot of lung tissue (n=3 in each group). Full-length blots/gels were presented in Figure S1. TLR4, toll-like receptor 4; PM_2.5, particulate matter 2.5; p-, phospho-; NF-κB, nuclear factor-κB; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3; IL-1β, interleukin-1β; HMGB1, high mobility group box 1; OVA, ovalbumin; IHC, immunohistochemical.

In Figure 5, the NLRP3 immunohistochemical staining of the PM_2.5 48-hour group in Figure 5A was mistakenly replaced.

The original Figure 5 is presented below:

The correct version of Figure 5, containing the correct data for the NLRP3 immunohistochemical staining of PM_2.5 48-hour group in Figure 5A, is shown below.

Figure 5 IHC and western blot were used to measure the protein expression levels of CC16-treated mice after exposure to PM_2.5. (A) IHC staining (n=10 in each group). (B) Western blot of lung tissue (n=3 in each group). Full-length blots/gels were presented in Figure S2. TLR4, toll-like receptor 4; PM_2.5, particulate matter 2.5; CC16, club cell secretory protein 16; p-, phospho-; NF-κB, nuclear factor-κB; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3; IL-1β, interleukin-1β; HMGB1, high mobility group box 1; IHC, immunohistochemical.

In Figure 7, the cleaved IL-1β panels of the Western blot data in Figure 7B was incorrect.

The original Figure 7 is presented below:

The correct version of Figure 7, containing the correct data for the cleaved IL-1β western blot in Figure 7B, is shown below.

Figure 7 Western blot and RNA analysis of inflammatory and pyroptosis signaling pathways in BEAS-2B cells. (A-D) Western blot. (E) RNA analysis. Full-length blots/gels were presented in Figure S3. TLR4, toll-like receptor 4; NF-κB, nuclear factor-κB; p-, phospho-; HMGB1, high mobility group box 1; LPS, lipopolysaccharide; PM_2.5, particulate matter 2.5; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3; IL-1β, interleukin-1β.

In Figure 8, the merged confocal image of the 0.25 μg/mL CC16 group in Figure 8B was incorrect.

The original Figure 8 is presented below:

The correct version of Figure 8, containing the correct data for the merged confocal image of the 0.25 μg/mL CC16 group in Figure 8B, is shown below.

Figure 8 Light microscopy, CCK-8 analysis, confocal microscopy, and electron microscopy to determine the viability of BEAS-2B cells treated with CC16. (A) Cell viability was inferred from CCK-8 analysis. (B) Confocal microscopy, Hoechst 33342 staining (blue), Annexin V-FITC (green), PI (red). (C) Electron microscopy. PM_2.5, particulate matter 2.5; CC16, club cell secretory protein 16; EHT, electron high tension; WD, working distance; Mag, magnification; SCUT, South China University of Technology; CCK-8, Cell Counting Kit-8; FITC, fluorescein isothiocyanate; PI, propidium iodide.

In Figure S2, the original Western blot image for TLR4 was inadvertently replaced with an incorrect raw data file during figure assembly.

The original Figure S2 is presented below:

The correct version of Figure S2, containing the correct data for the original Western blot image for TLR4, is shown below.

In addition, we noticed that there are some spelling errors in the figures and legends that need to be corrected:

• In Figures 3,5 , and Figure S2, “Gasdmin D” should be corrected as “Gasdermin D”;
• In Figures 3,5 , “P-NK-κB” should be corrected as “p-NF-κB”;
• In Figure 7, “NK-κB” “P-NK-κB” should be changed to “NF-κB” “p-NF-κB”;
• In the figure legend of Figure 8, “Hocest 33342 staining (blue)”, “EHT electron high yension” should be changed to “Hoechst 33342 staining (blue)” and “EHT, electron high tension”;
• In the figure legend of Figure S2, “cascade” should be corrected as “caspase”.

The authors regretted the errors and confirmed they would not change the results or conclusions of the article.

Click here to view the updated version of the article.

Figure S2 Western blot analysis of protein expression in various treatment groups. Lanes 1–4 represent control group, LPS (1 μg/mL) group, PM_2.5 (100 μg/mL) group, and PM_2.5 (200 μg/mL) group, respectively. The blots were probed with anti-NLRP3/caspase-1/cleaved caspase-1/gasdermin D/IL-1β/cleaved IL-1β/TLR4/NF-κB/p-NF-κB/caspase-3/cleaved caspase-3/HMGB1 antibody (1:1,000 dilution), and β-actin was used as a loading control. Data are representative of three independent experiments.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/.

References

1. Lin J, Chen X, Chen Y, et al. Club cell secretory protein 16 promotes cell proliferation and inhibits inflammation and pyroptosis in response to particulate matter 2.5-induced epithelial damage in asthmatic mice. J Thorac Dis 2025;17:753-73. [Crossref] [PubMed]